This was shot with the 70-200 f4 handheld (no IS) at 1/25s F5.6 at 200mm
I was so excited when I first saw this tiger that I started shooting without changing the ISO. My 20D was in Av mode and initially I did not realize that the shutter speed was so slow. After I changed the ISO to 400, he moved closer to the fence, which became visible in all my later shots.