ISO 100. Taken through window glass. Hand held at 1/40 second, using "tele-macro" setting. 35mm focal length equivalent of 337mm. Stabilizer setting #2. Other in-camera settings: Contrast: +1; Sharpness: 0; Saturation: +1; Noise Reduction: 0. The "bee guards" on our hummingbird feeder do not work very well!