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Moth Lab - DNA Barcode of Life Project

Photos and descriptions of the methods used to photograph the moths.
The major activity since August 2008 has been participation in the DNA Barcode of Life for moths DNA , iBOL , BARCODE Bulletin and iBOL Blog
About 4745 of the specimens sent to Prof. Paul Hebert at the U. of Guelph, Ontario, Canada have been logged in.
There has been identification of 1033 species and the potential for another 130 or so that have distinct DNA but are yet to be identified.
My projects there are LPOKA, LPOKB, LPOKC, LPOKD and LPOKE -- Lepidoptera of Oklahoma - Bartlesville
I have found, so far, these species that show regional differences in their DNA sequence. More are expected as additional BOLD data is made public.
The Callopistria floridensis below is a very interesting case showing a wide local variation from Costa Rican specimens and a rare amino acid replacement in one of the local variants.
The Fissicrambus mutabilis information has recently been updated at BOLD to reflect the potential for four species.
It and the others show a bimodal distribution in the DNA divergence measure indicating regional variations or misidentified reference specimens in BOLD.
A 2% DNA divergence is a rough guide that indicates possible separate species might be among the specimens. There are some cases where less divergence exists among species.
One tree includes all BOLD data but no geographic information. The other includes my specimens and BOLD public data which includes geographic information.
As more data becomes public additional geographic information will be obtained. Also, interpretations might change with larger sample size.
Thanks to Bruce Walsh, Dan Janzen, J.-F. Landry and Bob Patterson for comments on various parts of this.
Relevant Reference: BC Study
Attracting with UV ( Blacklight )
Attracting with UV ( Blacklight )
Moth Prep
Moth Prep
Current Moth Photography Setup
Current Moth Photography Setup
Specimens for DNA Barcoding
Specimens for DNA Barcoding
Tree using local and public BOLD data
Tree using local and public BOLD data
Photos for BugGuide Forum
:: Photos for BugGuide Forum ::
Callopistria floridensis - OK vs. Costa Rica
:: Callopistria floridensis - OK vs. Costa Rica ::
Fissicrambus mutabilis - Four species  ?
:: Fissicrambus mutabilis - Four species ? ::
Dyseriocrania griseocapitella - 2 species?
:: Dyseriocrania griseocapitella - 2 species? ::
Acrolophus texanella - Two species
:: Acrolophus texanella - Two species ::
Antaeotricha schlaegeri  OK vs. TN and Canada
:: Antaeotricha schlaegeri OK vs. TN and Canada ::
Argyresthia alternatella OK vs. Canada
:: Argyresthia alternatella OK vs. Canada ::
Gnorimoschema gallaeasterella - 2 species?
:: Gnorimoschema gallaeasterella - 2 species? ::
Chionodes mediofuscella - East meets West
:: Chionodes mediofuscella - East meets West ::
Argyrotaenia velutinana - OK vs. Canada
:: Argyrotaenia velutinana - OK vs. Canada ::
Crambus saltuellus  OK vs. Canada
:: Crambus saltuellus OK vs. Canada ::
Decantha boreasella - Two species ?
:: Decantha boreasella - Two species ? ::
Episimus argutana - Two species ?
:: Episimus argutana - Two species ? ::
Agnippe prunifoliella - Two species
:: Agnippe prunifoliella - Two species ::
Grapholita packardi - Two species
:: Grapholita packardi - Two species ::
Paralobesia viteana - Local variations
:: Paralobesia viteana - Local variations ::
Sparganothis distincta - Two species
:: Sparganothis distincta - Two species ::
Gerdana caritella  OK vs TN
:: Gerdana caritella OK vs TN ::
Psilocorsis quercicella - 2 species
:: Psilocorsis quercicella - 2 species ::
Psilocorsis reflexella - 2 species
:: Psilocorsis reflexella - 2 species ::
Ancylis divisana - Two species
:: Ancylis divisana - Two species ::
Walshia miscecolorella - Three species?
:: Walshia miscecolorella - Three species? ::
Pseudotelphusa quercinigracella - 2 species
:: Pseudotelphusa quercinigracella - 2 species ::
Eucosma comatulana - Two species?
:: Eucosma comatulana - Two species? ::
Clepsis virescana - Two species?
:: Clepsis virescana - Two species? ::
Caloptilia stigmatella - OK vs. Canada
:: Caloptilia stigmatella - OK vs. Canada ::
Larisa subsolana - OK vs. TN
:: Larisa subsolana - OK vs. TN ::
Platynota idaeusalis - OK & TN vs. ON
:: Platynota idaeusalis - OK & TN vs. ON ::
Tosale oviplagalis
:: Tosale oviplagalis ::
Eucosma dorsisignatana - OK vs. MA
:: Eucosma dorsisignatana - OK vs. MA ::
Olethreutes baccatanum - 2 species
:: Olethreutes baccatanum - 2 species ::
Rhopobota finitimana - OK vs. Canada
:: Rhopobota finitimana - OK vs. Canada ::
Pococera asperatella - 2 species
:: Pococera asperatella - 2 species ::
Ethmia - Database Error
:: Ethmia - Database Error ::
Prionoxystus macmurtrei  OK vs. TN
:: Prionoxystus macmurtrei OK vs. TN ::
Aglossa cuprina  - OK and TN vs. Canada
:: Aglossa cuprina - OK and TN vs. Canada ::
Lithacodes fasciola - 2 species?
:: Lithacodes fasciola - 2 species? ::
Apoda y-inversum - OK vs TN
:: Apoda y-inversum - OK vs TN ::
Apoda biguttata  OK vs TN
:: Apoda biguttata OK vs TN ::
Euclea delphinii - 2 species
:: Euclea delphinii - 2 species ::
Anania coronata - Two species ?
:: Anania coronata - Two species ? ::
Herpetogramma thestealis - OK & TN vs BC
:: Herpetogramma thestealis - OK & TN vs BC ::
Pyrausta signatalis  OK vs NC
:: Pyrausta signatalis OK vs NC ::
Epipaschia superatalis - OK vs. TN
:: Epipaschia superatalis - OK vs. TN ::
Palpita quadristigmalis - Two species
:: Palpita quadristigmalis - Two species ::
Emmelina monodactyla - OK & ON vs. BC
:: Emmelina monodactyla - OK & ON vs. BC ::
Callizzia amorata - Two species
:: Callizzia amorata - Two species ::
Oreta rosea - Two species?
:: Oreta rosea - Two species? ::
Cisthene packardii  OK vs TN
:: Cisthene packardii OK vs TN ::
Grammia phyllira - Two species?
:: Grammia phyllira - Two species? ::
Apantesis phalerata
:: Apantesis phalerata ::
Apantesis Nais
:: Apantesis Nais ::
Pyrrharctia isabella
:: Pyrrharctia isabella ::
Amorpha juglandis
:: Amorpha juglandis ::
Xylophanes tersa - 2 species?
:: Xylophanes tersa - 2 species? ::
Protitame virginalis OK vs Canada
:: Protitame virginalis OK vs Canada ::
Glenoides texanaria - Local Variation
:: Glenoides texanaria - Local Variation ::
Euacidalia sericearia - Local variation
:: Euacidalia sericearia - Local variation ::
Lochmaeus bilineata - Local Variation
:: Lochmaeus bilineata - Local Variation ::
Heterocampa obliqua - Local Variation
:: Heterocampa obliqua - Local Variation ::
Furcula cinerea  Local variation
:: Furcula cinerea Local variation ::
Idia aemula
:: Idia aemula ::
Abagrotis alternata
:: Abagrotis alternata ::
Acronicta ovata - OK vs. TN
:: Acronicta ovata - OK vs. TN ::
Acronicta vinnula - OK vs. TN
:: Acronicta vinnula - OK vs. TN ::
Acronicta americana - Variations
:: Acronicta americana - Variations ::
Acronicta retardata - OK & TN vs. CN
:: Acronicta retardata - OK & TN vs. CN ::
Agnorisma badinodis -  OK vs. MA
:: Agnorisma badinodis - OK vs. MA ::
Orthosia alurina - OK vs. Canada
:: Orthosia alurina - OK vs. Canada ::
Baileya australis - OK and Canada vs. TN
:: Baileya australis - OK and Canada vs. TN ::
Baileya dormitans - OK & TN vs. CN
:: Baileya dormitans - OK & TN vs. CN ::
Lacinipolia renigera - OK & TN vs CN
:: Lacinipolia renigera - OK & TN vs CN ::
Rivula propinqualis  OK vs CN
:: Rivula propinqualis OK vs CN ::
Spargaloma sexpunctata - Two Species
:: Spargaloma sexpunctata - Two Species ::
Phalaenostola eumelusalis - 2 species?
:: Phalaenostola eumelusalis - 2 species? ::
Cerma cerintha
:: Cerma cerintha ::
Xanthorhoe lacustrata - 2 species
:: Xanthorhoe lacustrata - 2 species ::
Elaphria versicolor
:: Elaphria versicolor ::
Clemensia albata - 2 species?
:: Clemensia albata - 2 species? ::
Copivaleria grotei  OK vs CN
:: Copivaleria grotei OK vs CN ::
Harrisimemna trisignata
:: Harrisimemna trisignata ::
Eudryas unio - OK vs CN
:: Eudryas unio - OK vs CN ::
Orthonama obstipata - NA vs. Europe
:: Orthonama obstipata - NA vs. Europe ::
Raphia frater  - OK vs. Canada
:: Raphia frater - OK vs. Canada ::
Agrotis gladiaria - OK vs. Canada
:: Agrotis gladiaria - OK vs. Canada ::
Feltia jaculifera - OK vs. CN
:: Feltia jaculifera - OK vs. CN ::
Tortricidia
:: Tortricidia ::
1134.1  Spinitibia hodgesi
:: 1134.1 Spinitibia hodgesi ::
Probole Taxonomy Tree 4/2012
Probole Taxonomy Tree 4/2012
MD052711-6 cc.jpg
MD052711-6 cc.jpg