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The name Basilica of Guadalupe (also Basilica of Our Lady of Guadalupe, Basílica de Nuestra Señora de Guadalupe in Spanish) may refer to one of the two churches built on top of Tepeyac hill, north of Mexico City. The site is nearby the place where it is said Our Lady of Guadalupe appeared in front of Juan Diego Cuauhtlatoatzin. This site is also known as La Villa de Guadalupe or, in a more popular sense, simply La Villa.

The new Basilica houses the original tilma (or apron) of Juan Diego that shows the icon of Our Lady of Guadalupe. Thus it is an important pilgrimage site and is visited by several million people every year, especially around December 12, Our Lady of Guadalupe's Feast day.

The Basilica is part of the Historic Centre of Mexico City, which was added to the UNESCO World Heritage List in 1987.